DNA

Part:BBa_K156002:Design

Designed by: George McArthur IV   Group: iGEM08_Virginia   (2008-10-27)

BioBrick placeholder 2 (Prefix - ApoI (NotI) NheI - Suffix)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 43
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NheI site found at 37
    Illegal SpeI site found at 44
    Illegal PstI site found at 58
    Illegal NotI site found at 7
    Illegal NotI site found at 29
    Illegal NotI site found at 51
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 44
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 44
    Illegal PstI site found at 58
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sites are compatible with standard BBa restriction sites and will form scars when ligated.


Source

The DNA sequence is simply linked restriction enzyme sites that are compatible with standard BBa restriction sites. These compatible sites were compiled and listed in "Engineering BioBrick vectors from BioBrick parts". http://www.jbioleng.org/content/2/1/5

References

Shetty RP, Endy D, Knight TF Jr. (2008). Engineering BioBrick vectors from BioBrick parts. J Biol Eng 2: 5.